[Hydrolase activity characteristics of actinomycetes during antibiotic biosynthesis]

The activity of the main extracellular hydrolytic enzymes, such as protease, nuclease and amylase was studied in the process of the development of the actinomycetes producing streptomycin, novobiocin, levorin and oleandomycin. It was found that the enzymatic activity significantly changed during the development of the cultures depending on their individual properties. Comparative study of the enzymatic activity of the streptomycin-producing organisms differing in the antibiotic production levels and the use of special nutrient media providing the increased biosynthesis of DNAases allowed a definite relationship to be disclosed between the activity level of extracellular hydrolases and antibiotic production.