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Membrane potentials in yeast cells measured by direct and indirect methods
Biochimica Et Biophysica Acta
|April 22, 1981
Abstract:
The membrane potential, delta psi, of various yeasts estimated from the distribution of tetraphenylphosphonium cations ranged from -50 to -120 mV, depending on species, incubation conditions and technique of measurement. Values obtained directly with a microelectrode in Endomyces magnusii were consistently lower than those determined indirectly.
Insights
The membrane potential (delta psi) in yeasts varies widely, from -50 to -120 mV. Direct microelectrode measurements in Endomyces magnusii yielded lower values than indirect methods.
Area of Science:
- Microbiology
- Cell Physiology
- Biophysics
Background:
- The membrane potential (delta psi) is a critical indicator of cellular function.
- Accurate measurement of delta psi is essential for understanding yeast physiology.
Purpose of the Study:
- To estimate the membrane potential (delta psi) in various yeast species.
- To compare indirect estimation methods with direct microelectrode measurements.
Main Methods:
- Utilized tetraphenylphosphonium cation distribution for indirect delta psi estimation.
- Employed microelectrode impalement for direct delta psi measurement in Endomyces magnusii.
Main Results:
- Estimated delta psi values ranged from -50 to -120 mV across different yeast species.
- Direct microelectrode measurements in Endomyces magnusii consistently showed lower delta psi values compared to indirect methods.
Conclusions:
- Yeast membrane potential is highly variable, influenced by species, incubation, and measurement technique.
- Direct microelectrode measurements provide a more accurate assessment of delta psi in certain yeast species.