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Membrane potentials in yeast cells measured by direct and indirect methods

V Vacata, A Kotyk, K Sigler

    Biochimica Et Biophysica Acta
    |April 22, 1981
    PubMed
    Summary

    The membrane potential (delta psi) in yeasts varies widely, from -50 to -120 mV. Direct microelectrode measurements in Endomyces magnusii yielded lower values than indirect methods.

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    Area of Science:

    • Microbiology
    • Cell Physiology
    • Biophysics

    Background:

    • The membrane potential (delta psi) is a critical indicator of cellular function.
    • Accurate measurement of delta psi is essential for understanding yeast physiology.

    Purpose of the Study:

    • To estimate the membrane potential (delta psi) in various yeast species.
    • To compare indirect estimation methods with direct microelectrode measurements.

    Main Methods:

    • Utilized tetraphenylphosphonium cation distribution for indirect delta psi estimation.
    • Employed microelectrode impalement for direct delta psi measurement in Endomyces magnusii.

    Main Results:

    • Estimated delta psi values ranged from -50 to -120 mV across different yeast species.
    • Direct microelectrode measurements in Endomyces magnusii consistently showed lower delta psi values compared to indirect methods.

    Conclusions:

    • Yeast membrane potential is highly variable, influenced by species, incubation, and measurement technique.
    • Direct microelectrode measurements provide a more accurate assessment of delta psi in certain yeast species.

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