Sephadex G-10 columns do not retain selectively T or B lymphocyte subpopulations

The passage through Sephadex G-10 columns of lymphocytes prepared from different lymphoid compartments of man, guinea pig and rabbit, did not result in a selective loss of lymphocyte subsets, as assessed by various rosetting assays. This lack of T and B lymphocyte loss was seen irrespective of whether glass beads or a fine mesh net was used as a support for the Sephadex G-10 column.