Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An automatic multi-programmed affinity chromatographic system

J Folkersen, B Teisner, J Westergaard

    Journal of Immunological Methods
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Hypersensitivity to intravenous iron: classification, terminology, mechanisms and management.

    British journal of pharmacology·2015
    Same author

    Commentary: antibodies reacting with the infliximab Fab portion--something new? Authors' reply.

    Alimentary pharmacology & therapeutics·2013
    Same author

    Pre-existing IgG antibodies cross-reacting with the Fab region of infliximab predict efficacy and safety of infliximab therapy in inflammatory bowel disease.

    Alimentary pharmacology & therapeutics·2013
    Same author

    Aggressive periodontitis in a 16-year-old Ghanaian adolescent, the original source of Actinobacillus actinomycetemcomitans strain HK1651 - a 10-year follow up.

    International journal of paediatric dentistry·2006
    Same author

    Dlk1 in normal and abnormal hematopoiesis.

    Leukemia·2005
    Same author

    Placental protein 14 concentrations in circulation related to hormonal parameters and reproductive outcome in women undergoing IVF/ICSI.

    Reproductive biomedicine online·2004
    Same journal

    Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.

    Journal of immunological methods·2026
    Same journal

    Isosulfan blue sentinel lymph node biopsy enables reliable lymph node harvest and multicolor flow cytometry in mice.

    Journal of immunological methods·2026
    Same journal

    Type-specific antibody detection of herpes simplex virus types 1&2 (HSV-1&2) in fingerstick blood at point-of-care sites by a rapid and sensitive lateral flow immunochromatographic assay.

    Journal of immunological methods·2026
    Same journal

    Development of chimeric DGP-IgG antibodies as quality control for celiac disease diagnosis.

    Journal of immunological methods·2026
    Same journal

    Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

    Journal of immunological methods·2026
    Same journal

    Performance evaluation of Meso Scale Discovery (MSD) quantitative serological assays for detection of binding (IgG, IgA, IgM) and ACE2 inhibitory antibody levels for SARS-CoV-2.

    Journal of immunological methods·2026
    See all related articles

    A new automated immunospecific affinity-chromatography system enables continuous operation for protein purification. This system simplifies complex processes like pregnancy zone protein isolation using immunosorbent gel, improving efficiency.

    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Biotechnology

    Background:

    • Affinity chromatography is a powerful technique for biomolecule purification.
    • Continuous operation systems can enhance efficiency and throughput in chromatographic processes.
    • Automated systems reduce manual labor and potential for error.

    Purpose of the Study:

    • To describe a novel automatic immunospecific affinity-chromatographic system for continuous operation.
    • To demonstrate the system's capability in purifying specific proteins.
    • To showcase the integration of sample application, washing, elution, and dialysis steps.

    Main Methods:

    • Development of an automated system with time-controlled steps for sample application, washing, and elution.
    • Integration of automatic dialysis for eluted fractions.

    Related Experiment Videos

  • Utilized immunosorbent gel for affinity purification.
  • Purification of pregnancy zone protein as a model application.

    • Main Results:

    • Successful implementation of a continuous operation automatic immunospecific affinity-chromatographic system.
    • Demonstrated effective purification of pregnancy zone protein.
    • The system integrates multiple chromatographic and post-chromatographic steps automatically.

    Conclusions:

    • The described system offers an efficient and automated approach for continuous immunospecific affinity chromatography.
    • This automation is applicable to the purification of various biomolecules, exemplified by pregnancy zone protein.
    • The integrated dialysis feature enhances the utility of the system for downstream applications.