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Summary
Histamine in brain capillaries regulates vascular permeability. Studies show histamine enhances endothelial cell pinocytosis and prostaglandin synthesis, influencing brain microvessel function.
Area of Science:
- Neuroscience
- Vascular Biology
- Biochemistry
Background:
- Histamine affects peripheral vascular permeability.
- The role of histamine in the cerebral vasculature was previously unclear.
Purpose of the Study:
- To investigate the presence and function of histamine in brain capillaries.
- To determine histamine's effect on cerebral microvascular permeability.
Main Methods:
- Subcellular fractionation to isolate brain capillaries.
- Intracarotid histamine infusion in animal models.
- Intracerebral ventricular injection of [3H]-histamine.
- Measurement of hydroxyfatty acid and prostaglandin D2 synthesis.
Main Results:
- High histamine levels were detected in isolated brain capillaries.
- Histamine infusion increased endothelial cell pinocytosis and astrocytic swelling.
- [3H]-Histamine localized to the capillary wall.
- Histamine and cimetidine stimulated hydroxyfatty acid and prostaglandin D2 synthesis.
Conclusions:
- Histamine is present in brain capillaries and influences cerebral microvascular permeability.
- Capillary histamine plays a role in regulating the brain's microvasculature.
- Histamine signaling in brain capillaries impacts prostaglandin synthesis.