Alloimmune neonatal thrombocytopenia: platelet antibody detection using the indirect immunofluorescence test (IIFT)

Two cases of neonatal alloimmune thrombocytopenia are presented. Using the indirect immunofluorescence test on platelets in suspension, the presence of non-agglutinating platelet specific antibodies with the specificity anti-Zwa could be demonstrated. In both cases diagnosis of alloimmunization was made before delivery of a second child, typing of the family was done and recommendations could be made concerning clinical management. Compatibility testing between the mother's serum and donor platelets (Zwa-negative) was predictable of a normal transfused platelet survival in the newborn.