Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural interaction of cytoskeletal components

M Schliwa, J van Blerkom

    The Journal of Cell Biology
    |July 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The (t)WE lab Simplified IVF Procedure: First Births after freezing/thawing.

    Facts, views & vision in ObGyn·2014
    Same author

    Unusual tubulins for unusual cells.

    Protist·2012
    Same author

    Calibration of light forces in optical tweezers.

    Applied optics·2010
    Same author

    Dictyostelium centrin-related protein (DdCrp), the most divergent member of the centrin family, possesses only two EF hands and dissociates from the centrosome during mitosis.

    European journal of cell biology·2001
    Same author

    Structure of a fast kinesin: implications for ATPase mechanism and interactions with microtubules.

    The EMBO journal·2001
    Same author

    Unusual properties of the fungal conventional kinesin neck domain from Neurospora crassa.

    The EMBO journal·2001

    The cytoskeleton is a highly interconnected structural unit, featuring novel 2-3nm filaments that link various cytoskeletal elements. Microtubule bending is influenced by interactions with other cytoskeletal components, forming a complex cellular backbone.

    Area of Science:

    • Cell Biology
    • Cytoskeletal Dynamics
    • Electron Microscopy

    Background:

    • The three-dimensional organization of the cytoskeleton is crucial for cellular structure and function.
    • Previous studies have provided insights into cytoskeletal networks, but detailed structural continuity remained unclear.

    Purpose of the Study:

    • To investigate the three-dimensional organization and interconnections of cytoskeletal filaments in detergent-treated cells.
    • To identify novel cytoskeletal components and their roles in structural organization.

    Main Methods:

    • High-voltage electron microscopy of whole-mount detergent-extracted African green monkey kidney (BSC-1) cells and chick embryo fibroblasts.
    • Stereo imaging at low and high magnifications.
    • Improved extraction procedures combined with heavy meromyosin subfragment 1 (S1) decoration of actin filaments.

    Related Experiment Videos

    Main Results:

    • Demonstration of a highly interconnected cytoskeletal unit.
    • Identification of a new class of 2- to 3-nm diameter filaments acting as linkers between various cytoskeletal elements (actin, intermediate filaments, microtubules).
    • Observation of end-to-side contacts formed by actin filaments with other cytoskeletal components, influencing microtubule course and causing bends.

    Conclusions:

    • The cytoskeleton is a complex, interconnected structural unit, not merely a collection of individual filament types.
    • Novel 2-3nm filaments and actin filament interactions contribute significantly to cytoskeletal organization and dynamics.
    • Microtubule organization is actively influenced by interactions with other cytoskeletal elements, suggesting a dynamic regulatory role.