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Related Experiment Videos

Modified oxidase and benzidine tests for separation of staphylococci from micrococci

A Faller, K H Schleifer

    Journal of Clinical Microbiology
    |June 1, 1981
    PubMed
    Summary

    Two rapid tests, modified oxidase and benzidine, differentiate staphylococci from micrococci. These methods are crucial for accurate bacterial identification in clinical settings.

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    Systematic and applied microbiology·2012

    Area of Science:

    • Microbiology
    • Bacteriology

    Background:

    • Accurate differentiation between staphylococci and micrococci is essential for clinical and research microbiology.
    • Existing methods for separation can be time-consuming or complex.

    Purpose of the Study:

    • To describe two simple and rapid methods for distinguishing staphylococci from micrococci.
    • To evaluate the efficacy of modified oxidase and benzidine tests for this purpose.

    Main Methods:

    • Utilized a modified oxidase test with tetramethylphenylenediamine in dimethyl sulfoxide.
    • Employed a benzidine test to detect c-type cytochromes after heme group removal.
    • Tested overnight cultures on blood agar for optimal results.

    Main Results:

    • Micrococci and *Staphylococcus sciuri* produced a blue color with the oxidase test, while other staphylococci did not.
    • The benzidine test confirmed the presence of c-type cytochromes in micrococci and *S. sciuri*.
    • Strict adherence to nutritional requirements and incubation times enhanced test accuracy.

    Conclusions:

    • The modified oxidase test offers a simple and rapid method for separating most staphylococci from micrococci.
    • The benzidine test aids in identifying specific bacterial groups based on cytochrome presence.
    • These tests are valuable for rapid identification in clinical material, particularly when *S. sciuri* is not anticipated.

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