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Rapid oxidase method for testing oxidase-variable Aeromonas hydrophila strains
Journal of Clinical Microbiology
|June 1, 1981
Summary
A new rapid oxidase test method prevents false-negative results for Aeromonas hydrophila. This technique provides same-day results within 3 hours for rapidly growing gram-negative rods.
Area of Science:
- Clinical microbiology
- Bacteriology
Background:
- False-negative oxidase reactions can occur with Aeromonas hydrophila.
- Traditional methods require extended incubation periods, delaying identification.
Purpose of the Study:
- To describe a rapid, same-day oxidase test procedure.
- To overcome false-negative oxidase reactions in Aeromonas hydrophila.
- To reduce identification time for gram-negative rods.
Main Methods:
- A novel oxidase test procedure was developed.
- The method is applicable to bacteria cultured on differential media like MacConkey agar.
- Testing is performed on colonies removed from the agar surface.
Main Results:
- The procedure obviates false-negative oxidase reactions.
- Oxidase testing can be completed within 3 hours.
- This rapid method is suitable for any rapidly growing gram-negative rod.
Conclusions:
- A rapid, same-day oxidase test is effective for Aeromonas hydrophila.
- This method significantly reduces the time required for bacterial identification.
- The procedure offers a valuable alternative for routine microbiological diagnostics.
