Related Experiment Video
Updated: Feb 3, 2026

Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis
Published on: September 26, 2025
Identification of Mycobacterium leprae: use of wall-bound mycolic acids
Abstract:
A simple method for extraction and analysis of wall-bound mycolic acids from small samples of mycobacteria is described. Separation of mycolic acid classes according to their functional groups by thin-layer chromatography showed a difference between Mycobacterium leprae and a number of strains of acid-fast bacilli cultured from leprosy biopsies in vitro. This technique is proposed as a convenient preliminary test in the identification of possible cultures of M. leprae.
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