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The direct radioimmunoassay technique for the determination of human alpha1-fetoprotein
Archivum Immunologiae Et Therapiae Experimentalis
|January 1, 1977
Abstract:
The direct radioimmunoassay procedure for the detection of human alpha-fetoprotein is described. The technique requires 20 microliter of sample for a single estimation and includes ammonium sulfate precipitation. The whole assay can be completed in 18 hours. AFP concentrations ranging from 2 ng/m1 to 500 ng/m1 can be quantified with reproducibility sufficient for clinical as well as experimental purposes.