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Updated: Aug 9, 2026
08:09
Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
Published on: November 16, 2015
Surface immunoglobulins of tonsil lymphocytes
Acta Microbiologica Academiae Scientiarum Hungaricae
|January 1, 1981
Summary
Human tonsillar lymphocytes bind immunoglobulins (IgG, IgA, IgM) at cold temperatures and release them when warmed. B cells show higher binding activity, with intrinsic lymphocyte determinants identified via immunofluorescence.
Area of Science:
- Immunology
- Cell Biology
Background:
- Lymphocytes play a crucial role in the immune system.
- Immunoglobulins (Ig) are key proteins involved in immune responses.
- Understanding Ig-lymphocyte interactions is vital for immunological research.
Purpose of the Study:
- To investigate the binding characteristics of human tonsillar lymphocytes to homologous immunoglobulins (IgG, IgA, IgM).
- To explore the role of B cells in immunoglobulin binding.
- To characterize the expression of intrinsic immunoglobulin determinants on lymphocyte surfaces.
Main Methods:
- Radiolabeling of human tonsillar lymphocytes with 14C-labelled homologous IgG, IgA, and IgM.
- Incubation at 5 degrees C for binding and 35 degrees C for release of immunoglobulins.
- Immunofluorescent staining to detect intrinsic immunoglobulin determinants on lymphocyte surfaces.
- Flow cytometry analysis to quantify cell populations.
Main Results:
- Human tonsillar lymphocytes demonstrated temperature-dependent binding and release of IgG, IgA, and IgM.
- B cells exhibited greater activity in immunoglobulin binding compared to other lymphocyte types.
- Preincubation at 35 degrees C reduced the labeling of lymphocytes with fluoresceinated anti-human Ig.
- Immunofluorescent staining revealed intrinsic immunoglobulin determinants on 16.0% IgG, 8.0% IgA, and 7.4% IgM-bearing cells.
Conclusions:
- Lymphocyte-immunoglobulin interactions are complex and influenced by temperature.
- B cells are significantly involved in the binding of immunoglobulins.
- The study provides insights into the demonstration of surface immunoglobulin determinants on lymphocytes, highlighting potential challenges due to weak binding.
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