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Bee venom specific human IgG was measured by solid phase sandwich radioimmunoassay (RIA) using bee venom adsorbed to polystyrene tubes. RIA results were found to correlate with those obtained in an enzyme immunoassay (EIA) for bee venom specific IgG. The RIA did not suffer from the high background often associated with other solid phase radioimmunoassays for human IgG. Furthermore, both the RIA and EIA were simple to perform, inexpensive and highly reproducible.
Bee venom specific human IgG was measured by solid phase sandwich radioimmunoassay (RIA) using bee venom adsorbed to polystyrene tubes. RIA results were found to correlate with those obtained in an enzyme immunoassay (EIA) for bee venom specific IgG. The RIA did not suffer from the high background often associated with other solid phase radioimmunoassays for human IgG. Furthermore, both the RIA and EIA were simple to perform, inexpensive and highly reproducible.