[Transfer of bone marrow microenvironment by stromal cells grown in culture and transplanted into sponges]

Monocellular suspensions from trypsinized CBA mouse bone marrow cells were explanted to monolayer cultures. The cells of the adhesive layer withdrawn from 15-30-day cultures were transplanted in gelatin sponges beneath the kidney capsule from syngeneic recipients. The transplantation was accompanied by bone formation and by transfer of bone marrow microenvironment in the overwhelming majority of cases.