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Sequential expression of B lymphocyte surface antigens in vitro
European Journal of Immunology
|May 1, 1981
Summary
This study reveals that B cells sequentially express surface antigens like Lyb-2, IgM, Ia, and IgD during differentiation in vitro. Inhibiting DNA synthesis allows simultaneous antigen expression, highlighting the role of cell division in this process.
Area of Science:
- Immunology
- Developmental Biology
- Cell Biology
Background:
- B cell differentiation involves the sequential expression of surface antigens.
- Understanding this process is crucial for comprehending immune system development and function.
Purpose of the Study:
- To investigate the sequential expression of B cell surface antigens in vitro.
- To determine the role of DNA synthesis in regulating antigen expression timing.
Main Methods:
- Utilized serological techniques and a 4-day in vitro culture system with neonatal mouse bone marrow lymphocytes.
- Employed E. coli lipopolysaccharide (LPS) for antigen induction and hydroxyurea (HU) to inhibit DNA synthesis.
- Assessed antigen induction via cytotoxicity assays, quantitative absorption, and protein A sheep red blood cell rosetting.
Main Results:
- B cells sequentially acquired Lyb-2, IgM, Ia, and IgD expression over 96 hours.
- Inhibition of DNA synthesis with HU allowed simultaneous induction of all antigens by LPS.
- Evidence suggests these antigens are expressed on the same differentiating B cell population.
Conclusions:
- A population of surface IgM-negative precursor B cells exists in neonatal bone marrow.
- In vitro culture and inhibition of DNA synthesis promote sequential antigen inducibility.
- The timing of antigen expression is linked to the cessation of DNA synthesis.