Related Experiment Video
Updated: Aug 12, 2026

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice
Published on: November 16, 2011
Short term regulation of mouse pyruvate dehydrogenase complex by insulin
Abstract:
Pyruvate dehydrogenase complex activity and its regulating factors were measured in diabetic mice in a starved or unstarved state. Both in starved and unstarved groups of normal mice, pyruvate dehydrogenase complex activity increased equally by insulin administration. On the other hand, in an unstarved group of diabetic mice, no change in pyruvate dehydrogenase complex activity was observed with the same treatment. With the starved group it increased within 10 minutes and recovered to the base value level in 30 minutes. As a result of studies on active form of pyruvate dehydrogenase complex in the liver and its regulating factors, it was found that no corresponding relation existed between the enzyme complex activity and its regulating factors. We found that unlike the normal mice, not only the previously known regulating mechanism but also other factors affected the pyruvate dehydrogenase complex activity in the diabetic mice.
Related Concept Videos
What is Glycolysis?
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
Cell Specific Gene Expression
Insulin Secretory Vesicles
PI3K/mTOR/AKT Signaling Pathway
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion
Insulin and C-peptide are co-secreted in...
Insulin: The Receptor and Signaling Pathways

