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Detection of plant virus by using purified IgG in ELISA
Journal of Virological Methods
|July 1, 1981
Abstract:
An optimized ELISA system for the detection of soybean mosaic virus was developed. Purified anti-soybean mosaic virus IgG, a secondary ovalbumin coat, and a clearly defined optimal concentration of enzyme-conjugated anti-soybean mosaic virus IgG are used. The test detects purified virus, or purified virus added to leaf and seed extracts, at a concentration of 2.5 ng/ml. Various buffers significantly affect detection sensitivity.