[Fixation of tissue specimens]

Authors studied the efficacy of various fixatives used in pathohistology. For general purposes and for impregnation techniques sodium-acetate-formalin, for the demonstration of cytoplasmic granula of endocrine cells and for staining of connective tissue elements Ort's solution and formalin with chrome-alum should be used. Demonstration of glycogen seems to be optimal after the use of the mixture of picric acid- acetic acid and alcohol as a fixative.