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Related Experiment Videos

Quantitation of tobramycin by solid-phase immunofluorescence

B Gerson, L Dean, F Bell

    Therapeutic Drug Monitoring
    |January 1, 1981
    PubMed
    Summary

    Solid-phase immunofluorescence offers a reliable method for quantifying tobramycin levels in patient sera. This technique demonstrates comparable precision and accuracy to radioimmunoassay, making it a viable alternative for clinical laboratories.

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    Area of Science:

    • Clinical Chemistry
    • Pharmacology
    • Analytical Toxicology

    Background:

    • Aminoglycoside antibiotics like tobramycin require careful therapeutic drug monitoring to prevent toxicity.
    • Accurate quantification of tobramycin in patient serum is crucial for optimizing dosage and patient outcomes.
    • Existing methods for tobramycin quantitation may have limitations in terms of cost, accessibility, or turnaround time.

    Purpose of the Study:

    • To evaluate the performance of solid-phase immunofluorescence as an alternative method for tobramycin quantitation.
    • To compare the accuracy and precision of solid-phase immunofluorescence with radioimmunoassay for tobramycin measurement.
    • To assess potential interference from other antibiotics, such as gentamicin.

    Main Methods:

    • Tobramycin concentrations were measured in serum samples from 81 patients using both radioimmunoassay (RIA) and solid-phase immunofluorescence (SPIF).

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  • Statistical analysis was performed to compare the bias, precision (within-run and run-to-run), and correlation between the two methods.
  • Potential interference from gentamicin was specifically investigated.

    • Main Results:

    • No statistically significant or clinically relevant difference was observed between tobramycin quantitation by RIA and SPIF (bias = 0.006 µg/mL, r = 0.97).
    • Within-run and run-to-run precision were comparable for both analytical methods.
    • No interference from gentamicin was detected, indicating method specificity.

    Conclusions:

    • Solid-phase immunofluorescence is a valid and comparable alternative to radioimmunoassay for the therapeutic drug monitoring of tobramycin.
    • This method offers a potentially accessible and accurate option for tobramycin quantitation in clinical laboratory settings.
    • Further validation in diverse laboratory environments may confirm its broader applicability.