Characterization of tilorone-induced mouse migration inhibitory factor by affinity chromatography and isoelectric...

Administration of tilorone to mice highly reproducibly induces a nontoxic serum migration inhibitory factor (MIFt) which was previously shown to originate from macrophages. The inhibitory activity could partially be purified by affinity chromatography on alpha-L-fucose-Sepharose 6B columns. Isoelectric focusing demonstrated two separable chemical entities with IEPs of about 4.0 and 5.9. While the first constitutes the inducible MIFt, the latter apparently represents migration inhibitory serum factor being found also in normal mouse serum.