[Effect of insulin on carbohydrate metabolism in the catfish (Ictalurus melas). II) In vitro experiments]

Isolated hepatocyte preparations were used to study the "in vitro" effects of insulin on catfish (Ictalurus melas) carbohydrate metabolism. Insulin decreases spontaneous glycogen lowering either in absence or in presence of glucose in the medium. In the same time insulin produces a little increase on the cell glucose level, but reduces the cell uptake of glucose from medium. The effect of insulin shows to be less evident on isolated hepatocytes than in the intact animals.