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Updated: Jul 21, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

The induction of errors during in vitro DNA synthesis following chloroacetaldehyde-treatment of poly(dA-dT) and

J A Hall, R Saffhill, T Green

    Carcinogenesis
    |January 1, 1981
    PubMed

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    Proofreading01:43

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    Proofreading01:31

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    Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
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