Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Demonstration of particulate accumulations in solid tissue

M E LeFevre, H M Jablonski, J A Pile

    Experientia
    |July 15, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Quantitative imaging and microlocalization of boron-10 in brain tumors and infiltrating tumor cells by SIMS ion microscopy: relevance to neutron capture therapy.

    Cancer research·2001
    Same author

    Biodistribution of copper carboranyltetraphenylporphyrins in rodents bearing an isogeneic or human neoplasm.

    Journal of neuro-oncology·2001
    Same author

    Boron neutron capture therapy of brain tumors: functional and neuropathologic effects of blood-brain barrier disruption and intracarotid injection of sodium borocaptate and boronophenylalanine.

    Journal of neuro-oncology·2000
    Same author

    Long-term immunological memory in the resistance of rats to transplanted intracerebral 9L gliosarcoma (9LGS) following subcutaneous immunization with 9LGS cells.

    Journal of neuro-oncology·2000
    Same author

    Boron neutron capture therapy of brain tumors: enhanced survival and cure following blood-brain barrier disruption and intracarotid injection of sodium borocaptate and boronophenylalanine.

    International journal of radiation oncology, biology, physics·2000
    Same author

    Effect of dose and infusion time on the delivery of p-boronophenylalanine for neutron capture therapy.

    Journal of neuro-oncology·1999

    This study introduces a new tissue clearing method using pancreatin for better visualization. It helps track long-term particle redistribution in mouse organs, improving the detection of hard-to-find particles.

    Area of Science:

    • Histology
    • Biomedical Imaging
    • Particle Toxicology

    Background:

    • Studying long-term particle distribution in tissues is challenging with traditional methods.
    • Locating large, infrequent particles within complex biological matrices requires advanced techniques.

    Purpose of the Study:

    • To describe a novel histological clearing procedure using partial tissue hydrolysis.
    • To demonstrate the application of this method for studying particle redistribution in vivo.
    • To evaluate the utility of the method for locating difficult-to-find particles.

    Main Methods:

    • Developed a histological clearing technique involving partial tissue hydrolysis with pancreatin.
    • Quantitated changes in the distribution of 5.7-micrometer latex particles in mouse liver and heart muscle.

    Related Experiment Videos

  • Analyzed particle distribution 6 months after intravenous latex injection.

    • Main Results:

    • The pancreatin-based clearing method allows for effective visualization of particle distribution.
    • Long-term redistribution patterns of latex particles in mouse liver and heart muscle were successfully studied.
    • The technique proved useful for locating large, infrequent particles that are difficult to detect using standard tissue sectioning.

    Conclusions:

    • Partial tissue hydrolysis with pancreatin is an effective histological clearing method.
    • This technique enhances the study of long-term in vivo particle redistribution.
    • The method improves the ability to locate large, infrequent particles in biological tissues.