Related Experiment Video
Updated: Aug 13, 2026

An In Ovo Model for Testing Insulin-mimetic Compounds
Published on: April 23, 2018
[A2-Norleucine]insulin. An analog with unanticipated biological properties
Abstract:
The simple replacement of the A2-isoleucine by norleucine in the insulin molecule gave an analog [A2-norleucine]insulin ([Nle A2]-insulin) which was found to possess insignificant receptor-binding affinity and in vitro biological activity, 0.6 and 0.9 percent, respectively, compared to the natural hormone. Circular dichroic studies showed that this analog is monomeric and indicated that the helical segment A2-A8 and the beta strand B24-B29 have undergone conformational changes. The reduced receptor-binding affinity of [Nle A2]insulin is attributed to the distortion of the A2-A8 helical segment which in turn may lead to the displacement of the A1, A5 and A19 residues which are putatively involved in receptor binding.
Related Concept Videos
Insulin: Biosynthesis, Chemistry, and Preparation
Damage or functional impairment of β-cells inhibits insulin production, leading to diabetes. Diabetes treatment primarily uses...
Insulin Formulations: Types and Delivery
Short-acting insulins are divided into rapid-acting...
Insulin: Dosing Regimen and Adverse Effects
The basal dose constitutes about 40%-50% of the total daily dose, with the rest as premeal insulin. The mealtime insulin dose should mirror...
Oral Hypoglycemic Agents: Sulfonylureas
Oral Hypoglycemic Agents: Glinides
Glucagon-like Receptor Agonists
GLP-1, when administered in high doses intravenously, triggers insulin secretion, inhibits glucagon release, slows gastric emptying, reduces food intake, and restores normal insulin secretion. However, its rapid inactivation by the...

