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Positive immunoselection using antibody-enzyme complexes

E Lakow, R S Basch

    Journal of Immunological Methods
    |January 1, 1981
    PubMed
    Summary
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    Researchers developed a novel immunoselection method using antibody-enzyme complexes to protect target cells from hydrogen peroxide (H2O2) damage. This technique enables the isolation of specific cells for various research applications.

    Area of Science:

    • Immunology
    • Cell Biology
    • Biotechnology

    Background:

    • Hydrogen peroxide (H2O2) can cause lethal damage to cells.
    • Targeting specific cell surface antigens is crucial for cell isolation and manipulation.
    • Existing methods for cell selection may have limitations in efficiency and specificity.

    Purpose of the Study:

    • To develop an immunoselection technique for protecting antigen-positive target cells from H2O2.
    • To establish a method for identifying and isolating cells based on specific antigen expression.
    • To demonstrate the efficacy of antibody-enzyme complexes in cell protection and selection.

    Main Methods:

    • Development of catalase-anti-catalase complexes coupled to specific antibodies.
    • Utilizing an antibody bridge for binding complexes to target cells (e.g., Thy-1 positive cells).

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  • Dual radiolabeling to quantify the amount and composition of bound complexes.

    • Main Results:

    • Successful protection of Thy-1 positive cells from H2O2 using the developed immunoselection technique.
    • Demonstration of antibody-enzyme complex adherence to target cells via an antibody bridge.
    • Quantification of bound complexes, validating the binding mechanism.

    Conclusions:

    • The immunoselection technique effectively protects antigen-positive cells from H2O2.
    • This method allows for the identification and isolation of specific cell populations.
    • The technique is broadly applicable for any antigen with available specific antisera.