Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Three-dimensional microanatomy of intracellular structures

K Tanaka, T Kinose, K Atoh

    Progress in Clinical and Biological Research
    |January 1, 1981
    PubMed
    Summary

    The Osmium-DMSO-Osmium method enabled clear 3D visualization of cell organelles, including endoplasmic reticulum, mitochondria, Golgi complex, and centrioles, revealing detailed structures like ribosomes and inner membrane particles.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Spatial distribution of nociceptive neuropeptide and nerve growth factor depletion in experimental diabetic peripheral nervous system.

    The Journal of international medical research·2002
    Same author

    Impaired tetramer assembly of variant medium-chain acyl-coenzyme A dehydrogenase with a glutamate or aspartate substitution for lysine 304 causing instability of the protein.

    The Journal of biological chemistry·1992
    Same author

    The prognostic value of cytogenetic analyses in patients with acute nonlymphocytic leukemia treated with the same intensive chemotherapy.

    Cancer·1992
    Same author

    Ornithine decarboxylase is degraded by the 26S proteasome without ubiquitination.

    Nature·1992
    Same author

    [Antitumor effect of locally injected TNF.fibrin gel against murine colon 26 subcutaneous tumor: preliminary report].

    Nihon Geka Gakkai zasshi·1992
    Same author

    [Clinical studies on cefprozil granules in pediatric skin soft tissues infections].

    The Japanese journal of antibiotics·1992

    Area of Science:

    • Cell Biology
    • Microscopy
    • Ultrastructure

    Background:

    • Understanding the intricate three-dimensional structure of cellular organelles is crucial for elucidating their functions.
    • Traditional preparation methods can introduce artifacts, limiting detailed ultrastructural analysis.

    Purpose of the Study:

    • To present a novel sample preparation technique for high-resolution three-dimensional imaging of cell organelles.
    • To detail the ultrastructural features of key organelles using this new method.

    Main Methods:

    • Specimen preparation using the "Osmium-DMSO-Osmium method" for three-dimensional observation.
    • High-resolution imaging of cellular organelles.

    Main Results:

    • Detailed 3D visualization of endoplasmic reticulum cisternae with studded ribosomes.
    • Clear observation of mitochondrial cristae with potential inner membrane particles.
    • Characterization of the Golgi complex, including vesicles, tubules, and vacuoles.
    • Identification of centrioles as cylindrical bodies with tubular elements and granular contents.

    Conclusions:

    • The Osmium-DMSO-Osmium method provides superior three-dimensional ultrastructural detail of cell organelles.
    • This technique facilitates a deeper understanding of organelle morphology and potential functional components.

    Related Experiment Videos