Three-dimensional microanatomy of intracellular structures

Cell organellae were observed in three-dimensions with specimens prepared by the "Osmium-DMSO-Osmium method". 1. Cisternae of the endoplasmic reticulum were closely apposed to form a lamellar system of flat cavities. On their outer surfaces, numerous ribosomes were studded. 2. In mitochondria, the cristae mitochondriales were clearly observed. On the cristae, numerous small particles, which might correspond to inner membrane particles, were seen. 3. Golgi complex consisted of a Golgi stack with numerous vesicles, a plexus of anastomotic tubuli, and vacuoles which might be secondary lysosomes. 4. Centrioles was observed as a cylindric body. Its wall might be composed of a bundle of tubular elements and the central cavity was filled up with small granules.