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Related Experiment Videos

Effect of antimicrobial agents on human polymorphonuclear leukocyte microbicidal function

W D Welch, D Davis, L D Thrupp

    Antimicrobial Agents and Chemotherapy
    |July 1, 1981
    PubMed
    Summary

    Certain antibiotics, including tetracycline, trimethoprim, and cephalothin, may impair the infection-fighting ability of human polymorphonuclear leukocytes (PMNs) at therapeutic doses. This reversible inhibition affects key functions like yeast killing.

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    Area of Science:

    • Immunology
    • Pharmacology
    • Microbiology

    Background:

    • Human polymorphonuclear leukocytes (PMNs) are critical for innate immunity.
    • Antimicrobial agents are widely used, and their potential impact on host immune cells requires investigation.

    Purpose of the Study:

    • To evaluate the effects of 19 antimicrobial agents on human PMN function.
    • To determine if common antimicrobial drugs interfere with PMN microbicidal activity.

    Main Methods:

    • Chemiluminescence assays were used to measure PMN activation.
    • Yeast phagocytosis and killing assays assessed PMN's ability to engulf and destroy pathogens.
    • Lactate dehydrogenase release was measured to assess PMN cytotoxicity.

    Main Results:

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    • Tetracycline and trimethoprim inhibited PMN chemiluminescence and yeast killing at 2 microgram/ml.
    • Cephalothin reduced yeast killing at 20 microgram/ml and inhibited chemiluminescence at 200 microgram/ml.
    • Inhibitory effects were reversible, and no agents caused PMN cytotoxicity.

    Conclusions:

    • Therapeutic concentrations of tetracycline, trimethoprim, and cephalothin may reversibly inhibit optimal PMN microbicidal function.
    • Most tested antimicrobial agents did not affect PMN function at clinical serum levels.
    • Further research is needed to understand the clinical implications of these findings.