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Related Experiment Videos

Vimentin: a phosphoprotein under hormonal regulation

E T Browning, M M Sanders

    The Journal of Cell Biology
    |September 1, 1981
    PubMed
    Summary

    Researchers identified a 58K-5.7 protein in C-6 glioma cells as vimentin, a key intermediate filament protein. This finding advances understanding of cellular structure and protein function in glioma cells.

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    Area of Science:

    • Cell Biology
    • Neuroscience
    • Biochemistry

    Background:

    • Norepinephrine stimulation in C-6 glioma cells previously identified a 58K-5.7 protein as a cyclic AMP-dependent phosphoprotein.
    • This 58K-5.7 protein was found to be highly abundant in the nuclear fraction of these cells.

    Purpose of the Study:

    • To investigate the identity of the 58K-5.7 protein.
    • To determine if the 58K-5.7 protein is a vimentin-type intermediate filament protein.

    Main Methods:

    • Characterization of the 58K-5.7 protein's properties including size, isoelectric point, solubility, and association with nuclear structures.
    • Immunochemical analysis using antisera against hamster vimentin and the purified 58K-5.7 protein.
    • Techniques included immunoautoradiography and indirect immunofluorescence.

    Main Results:

    • The 58K-5.7 protein remained associated with the nuclear matrix after chromatin removal.
    • Immunoautoradiography confirmed selective reaction between anti-vimentin antisera and the 58K-5.7 protein in urea-solubilized cellular residues.
    • Indirect immunofluorescence demonstrated that antibodies against the 58K-5.7 protein bound to a fibrous cellular array characteristic of vimentin filaments.

    Conclusions:

    • The 58K-5.7 protein in C-6 glioma cells is identified as vimentin.
    • This protein is a vimentin-type intermediate filament protein.
    • The findings contribute to understanding the composition of nuclear structures and intermediate filaments in glioma cells.

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