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A miniaturised single-step method of cell cloning

C E Bishop

    Journal of Immunological Methods
    |January 1, 1981
    PubMed
    Summary

    This study presents a rapid, miniaturized cell cloning technique using Terasaki plates. This method simplifies screening and ensures accurate single-cell selection, reducing the need for multiple recloning steps.

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    Area of Science:

    • Cell Biology
    • Biotechnology

    Background:

    • Traditional cell cloning methods can be time-consuming and require multiple recloning steps.
    • Ensuring monoclonality is crucial for reliable experimental results.

    Purpose of the Study:

    • To introduce a miniaturized, rapid, and technically simple cell cloning method.
    • To leverage Terasaki plates for efficient screening and selection of single-cell clones.

    Main Methods:

    • Utilizing Terasaki plates, originally designed for tissue typing, for cell culture.
    • Screening wells to identify those containing a single cell.
    • Selecting colonies derived from wells with confirmed single-cell origin.

    Main Results:

    • The method allows for rapid testing of a large number of putative clones.
    • Terasaki plate wells facilitate precise observation of cell numbers.
    • Eliminates the need for secondary or tertiary recloning for statistical accuracy.

    Conclusions:

    • This miniaturized cell cloning technique offers a more efficient and reliable approach.
    • The use of Terasaki plates streamlines the process of identifying and selecting single-cell clones.

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