[Use of whole loose connective tissue preparations in immunofluorescence studies]

Evidence has been obtained for potential use of total loose connective tissue film preparations during immunofluorescence studies of antinuclear antibodies of serum from patients with systemic lupus erythematosus, antibodies to cytoplasmic components of fibroblasts of serum from patients with rheumatoid arthritis, antibodies to the surface fibroblast antigen fibronectin, and of certain regularities in IgG binding with mast cell components. As compared with other preparations (cryostat, paraffin sections) used in immunomorphology while applying the methods of labeled antibodies, the total preparations have the following basic advantages: simplicity of preparation and possibility to eliminate the denaturative effects on tissue structures.