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Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
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Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Published on: January 16, 2012

Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples

J B Olmsted

    The Journal of Biological Chemistry
    |December 10, 1981
    PubMed

    A method is described for the affinity purification of antibodies using protein samples that have been electrophoretically transferred to diazotized paper. Using differentiated neuroblastoma cells as the protein sample and a heterogeneous anti-microtubule protein serum, antibodies were isolated that specifically bound only to tubulin on blots and that stained microtubule networks in cells. The general utility of this method for various types of applications is discussed.

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