Frameshift mutations: relative roles of simple intercalation and of adduct formation

The contribution of "simple" intercalation and of adduct formation on the expression of frameshift mutations in Salmonella typhimurium was investigated using 9-aminoacridine and derivatives capable of either only intercalating between DNA base pairs or of forming adducts with DNA as well. For a chemical capable of intercalating as well as of forming an adduct, only a small portion of the frameshift mutagenicity is due to "simple" intercalation. Analogs only able to induce frameshift mutations as a result of intercalation generally display only a fraction (approx. 1%) of the frameshift activity of the analog capable of forming DNA adducts.