Subcellular distribution of inflammatory mediators in guinea-pig polymorphonuclear granulocytes (PMNL)

The experiments were performed for localizing the inflammation-inducing factors in the subcellular fractions of human and guinea pig PMNL. The magnitude of the induced inflammation tested on rats and guinea-pigs represented combined effects of all proteins extracted from PMNL. The isolation of PMNL subcellular components did not elevate the potency of the inflammatory response. The inflammation was completely abolished with SH-dependent protease inhibitor. The inflammation induced by PMNL factors is a complex phenomenon which could not be attributed to special subcellular components.