Oligodendrocytes of jimpy mice express galactosylceramide: an immunofluorescence study on brain sections and dissociated brain cell cultures

Brain sections and dissociated brain cell cultures of jimpy mouse (jp) were investigated for the presence of galactosylceramide (GC) by indirect immunofluorescence. Optic nerve and corpus callosum sections of 26-day-old jp exhibited many GC-positive cells. The GC staining pattern was similar in jp and normal cultures of the same age. These data suggest that the previously observed decreased amount of GC in jp brain is due to the inability of jp oligodendroglia to properly deposit GC in the myelin, while its synthesis is possible.