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Related Experiment Videos

Protease activities in human tears

P K Tsung, F J Holly

    Current Eye Research
    |January 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Human tear protease activity, including cathepsin B-like enzymes, is significantly higher in Orientals compared to Caucasians. This difference may stem from proteolytic products within the tears.

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    Area of Science:

    • Biochemistry
    • Ophthalmology
    • Human Physiology

    Background:

    • Proteases play crucial roles in biological processes.
    • Human tear composition can vary between ethnic groups.
    • Understanding tear protease activity is important for ocular health.

    Purpose of the Study:

    • To quantify and compare protease activities in human tears between different ethnic groups.
    • To investigate potential ethnic variations in specific protease levels.
    • To explore the origin of surface-active components in tears.

    Main Methods:

    • Measurement of several protease activities using six specific substrates in human tears.
    • Comparison of protease activity between Oriental and Caucasian individuals.
    • Evaluation of tear collection methods (glass capillary vs. filter-paper strip).

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  • Incubation of tears to assess the formation of surface-active fractions.

    • Main Results:

    • Cathepsin B-like enzyme activity was 7-fold higher in Orientals than in Caucasians.
    • Trypsin-like and cathepsin C-like enzyme activities were 3-4 fold higher in Orientals.
    • High variability in enzyme activity was observed within both ethnic groups.
    • Similar protease activity results were obtained regardless of the tear collection method.
    • A surface-active, low molecular weight fraction was produced after incubating tears.

    Conclusions:

    • Significant ethnic differences exist in human tear protease activities, particularly for cathepsin B-like, trypsin-like, and cathepsin C-like enzymes.
    • The observed differences suggest potential genetic or environmental factors influencing tear composition.
    • Proteolytic activity may contribute to the formation of surface-active substances in human tears.