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Biotyping and determination of protein A in Staphylococcus aureus strain of skin origin
Abstract:
By means of biotyping we have investigated 150 strains of Staphylococcus aureus isolated from people with dermatologic complaints. We have used six main and three additional biotyping tests. The strains under investigation did not form a closed biotype, on the contrary, they showed a certain biotype plurality. The determination of protein A in 150 strains of S. aureus isolated from dermatologic process of men, 15 strains of S. aureus of bovine origin and 50 strains of Staphylococcus with negative coagulase activity have brought the following results: all strains of S. aureus of skin origin (human strains) contain protein A in various quantities keeping within 2-64 U. Only 2 from 15 s. aureus strains of bovine origin contain protein A and there were none in 50 strains of Staphylococcus with a negative coagulase reaction.
Insights
This study examined Staphylococcus aureus strains from skin conditions. Human S. aureus strains consistently contain Protein A, unlike bovine strains or coagulase-negative Staphylococcus.
Area of Science:
- Microbiology
- Dermatology
- Bacteriology
Background:
- Staphylococcus aureus is a common cause of dermatologic complaints.
- Biotyping is crucial for differentiating bacterial strains.
- Protein A is a significant virulence factor in S. aureus.
Purpose of the Study:
- To biotype 150 strains of Staphylococcus aureus from human dermatologic conditions.
- To determine the presence and quantity of Protein A in these strains.
- To compare S. aureus strains of human origin with those of bovine origin and coagulase-negative Staphylococcus.
Main Methods:
- Utilized six main and three additional biotyping tests for bacterial characterization.
- Quantified Protein A levels in S. aureus strains using a defined unit (U).
- Included strains of human skin origin, bovine origin, and coagulase-negative Staphylococcus for comparative analysis.
Main Results:
- Human S. aureus strains from dermatologic conditions exhibited biotype plurality, not a uniform profile.
- All 150 human S. aureus strains contained Protein A, with quantities ranging from 2-64 U.
- Only 2 out of 15 bovine S. aureus strains contained Protein A; none of the 50 coagulase-negative Staphylococcus strains did.
Conclusions:
- Human S. aureus strains associated with skin infections possess Protein A, suggesting its role in dermatologic pathogenesis.
- The absence or low presence of Protein A in bovine S. aureus and coagulase-negative Staphylococcus differentiates them from human skin isolates.
- Biotyping and Protein A determination are valuable for distinguishing pathogenic S. aureus strains in clinical dermatology.