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Generation of Monoclonal Antibodies Against Natural Products
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[Preliminary serological analysis for identification of direct monoclonal antibodies against surface structures of

P Richiardi, T Crepaldi, A Amoroso

    Giornale Di Batteriologia, Virologia Ed Immunologia
    |July 1, 1980
    PubMed

    The following schedule has been applied to the selection and preliminary serological definition of monoclonal antibodies against HLA controlled antigenic structures of human lymphocyte surface: 1) identification of antibodies reactive against the immunizing cells by means of indirect radiobinding technique and complement-dependent lymphocytotoxicity; 2) analysis of the pattern of reaction against a panel of T and B lymphocytes purified from peripheral blood of HLA typed donors. The reaction has been performed also against lymphocytes pretreated with anti-human-beta 2 microglobulin antibodies. Thus monomorphic and polymorphic monoclonal antibodies, directed against beta 2 microglobulin associated or not associated antigens, can be identified. The importance of the complement pool and of antibody avidity in the lymphocytotoxicity reaction mediated by monoclonal antibodies, is evaluated.

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