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Simple method for isolation and presumptive identification of Clostridium difficile
Zentralblatt Fur Bakteriologie, Mikrobiologie Und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten Und Parasitologie = International Journal of Microbiology and Hygiene. A, Medical Microbiology, Infectious
|January 1, 1981
Summary
Clostridium difficile can be identified in stool samples using a specialized culture medium and UV light. Further confirmation involves enzyme testing and toxin detection to accurately diagnose infections.
Area of Science:
- Microbiology
- Infectious Diseases
- Diagnostic Techniques
Background:
- Clostridium difficile is a significant cause of antibiotic-associated diarrhea.
- Accurate and rapid laboratory identification is crucial for patient management.
Purpose of the Study:
- To describe a method for the presumptive and confirmatory identification of Clostridium difficile from stool samples.
Main Methods:
- Inoculation of stool onto chromid agar (CCCBA) followed by examination under UV light for characteristic golden-yellow fluorescent colonies.
- Identification of colonies using the API ZYM system.
- Confirmation of toxin production via tissue culture assay with Clostridium sordellii antitoxin.
Main Results:
- Golden-yellow fluorescent colonies on CCCBA under UV light indicate presumptive Clostridium difficile.
- API ZYM system provides further biochemical identification.
- Tissue culture confirms the presence of C. difficile toxins.
Conclusions:
- This combined approach allows for reliable presumptive and confirmatory identification of Clostridium difficile.
- The method aids in the diagnosis of C. difficile infections.