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ELISA: a tool for the clinical microbiologist

E M Peterson

    The American Journal of Medical Technology
    |November 1, 1981
    PubMed
    Summary

    Enzyme-linked immunosorbent assay (ELISA) offers versatile detection of antigens and antibodies. This review covers ELISA techniques, applications in clinical microbiology, and available commercial systems.

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    Area of Science:

    • Clinical Microbiology
    • Immunology
    • Biotechnology

    Background:

    • The enzyme-linked immunosorbent assay (ELISA) is a widely used immunoassay.
    • Its ability to detect both antigens and antibodies makes it valuable in diagnostics.
    • Applications in clinical microbiology are expanding.

    Purpose of the Study:

    • To review the fundamental principles and variations of ELISA techniques.
    • To explore the diverse applications of ELISA in clinical microbiology.
    • To discuss the availability of commercial ELISA materials and systems.

    Main Methods:

    • Description of basic ELISA procedures.
    • Explanation of technique variations.
    • Review of published applications in clinical microbiology.

    Main Results:

    • ELISA is increasingly utilized in clinical microbiology laboratories.
    • Various ELISA formats exist, offering flexibility.
    • A range of commercial kits and systems are available.

    Conclusions:

    • ELISA is a powerful tool for antigen and antibody detection in clinical microbiology.
    • Understanding ELISA techniques and applications is crucial for laboratory professionals.
    • The availability of commercial systems facilitates ELISA implementation.

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