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Related Experiment Videos

Conjugal transfer of R-plasmid R1drd-19 in Escherichia coli below 22 degrees C

P Singleton, A E Anson

    Applied and Environmental Microbiology
    |November 1, 1981
    PubMed
    Summary

    R-plasmids, like R1drd-19, can transfer between bacteria even below 22°C. This finding is crucial for understanding plasmid mobility in cooler environmental waters.

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    Area of Science:

    • Microbiology
    • Environmental Science
    • Genetics

    Background:

    • Conjugal transfer of R-plasmids is a key mechanism for antibiotic resistance dissemination.
    • Plasmid transfer is generally considered to be temperature-dependent, occurring above 22°C.

    Purpose of the Study:

    • To investigate the transferability of the R-plasmid R1drd-19 at temperatures below 22°C.
    • To assess the implications of low-temperature plasmid transfer for environmental waters.

    Main Methods:

    • Bacterial conjugation experiments were performed using strains carrying the R1drd-19 plasmid.
    • Transfer frequencies were measured at various temperatures, including those below 22°C.

    Main Results:

    • The R1drd-19 plasmid demonstrated successful conjugal transfer at temperatures below 22°C.
    • Transfer efficiency was observed to be lower at reduced temperatures but remained significant.

    Conclusions:

    • The established temperature threshold for R-plasmid transfer may not apply universally.
    • Low-temperature plasmid transfer has significant implications for the spread of antibiotic resistance genes in aquatic environments.

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