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Related Experiment Videos

Temporal changes in histone acetylation

M Petricevic, C W Denko, L Messineo

    Mechanisms of Ageing and Development
    |October 1, 1978
    PubMed
    Summary
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    Acetylation levels in rat thymus and liver change with age. Liver histone acetylation declines steadily, potentially linked to cell state changes, while thymus shows cyclical patterns.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Gerontology

    Background:

    • Acetylation is a key post-translational modification influencing protein function.
    • Understanding age-related changes in acetylation is crucial for cellular health.
    • Histones play a vital role in DNA packaging and gene regulation.

    Purpose of the Study:

    • To investigate the age-dependent incorporation of acetate into histones and other cellular fractions.
    • To compare acetylation patterns in different tissues (thymus, liver) and cellular compartments (cytoplasmic, histone) during aging.
    • To explore the relationship between liver histone acetylation and hepatocyte ploidy changes.

    Main Methods:

    • Administration of tritiated acetate to developing and aging rats.
    • Isolation of thymus, liver, serum, and cytoplasmic fractions.

    Related Experiment Videos

  • Extraction and radioactivity measurement of trichloroacetic acid-precipitable histone fractions from liver and thymus.
  • Quantification of radioactivity in serum and cytoplasmic fractions.

    • Main Results:

    • Serum acetate incorporation significantly declined with increasing age.
    • Thymus histone and cytoplasmic fractions exhibited a cyclic pattern of acetate incorporation.
    • Liver histone acetylation showed a consistent decline with age, stabilizing at a lower level.
    • The observed decline in liver histone acetylation is hypothesized to correlate with hepatocyte polyploidization.

    Conclusions:

    • Acetylation processes in rat liver and thymus are significantly affected by aging.
    • Age-related decline in liver histone acetylation may be associated with the transition of hepatocytes from diploid to polyploid states.
    • Cyclical acetylation patterns in the thymus suggest complex regulatory mechanisms during development and aging.