[Growth of continuous and primary cell cultures on a liquid surface]

A possibility to culture reinoculated BHK-21 cells and primary human embryo fibroblasts on the surface of liquid fluorocarbon is shown. Two types of growth of BHK cells on liquid surface can be distinguished: with the spreading of cells on the surface and formation of a monolayer, and with growth without cell spreading and with accumulation of cells in the form of clusters and small groups. Adhesion of cells to the liquid fluorocarbon surface is found to be weaker compared to the reproduction on the solid surface.