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Related Concept Videos

Ultrasonography01:17

Ultrasonography

8.4K
Ultrasonography is an imaging technique that uses high-frequency sound waves to visualize the body's internal structures. It is a non-invasive and safe procedure that does not involve the use of ionizing radiation, making it widely used in various medical fields. Ultrasonography is used to study heart function, blood flow in the neck or extremities, certain conditions such as gallbladder disease, and fetal growth and development.
During an ultrasonography procedure, a handheld device called...
8.4K

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Cortical Bone Assessment Using Ultrasonic Guided Waves: A Reproducibility Study in a Healthy Population
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Ultrasonic decalcification of bone

L Milan, M C Trachtenberg

    The American Journal of Surgical Pathology
    |September 1, 1981
    PubMed
    Summary

    Ultrasonic energization significantly speeds up bone decalcification to under 5 hours for 2-5 mm specimens. This method excellently preserves both hard and soft tissue morphology, enabling advanced histochemical analyses.

    Area of Science:

    • Histology
    • Biomaterials Science
    • Biotechnology

    Background:

    • Bone decalcification is crucial for histological analysis.
    • Traditional decalcification methods are time-consuming and can compromise tissue morphology.
    • Preserving tissue integrity is essential for accurate histochemical staining and downstream applications.

    Purpose of the Study:

    • To investigate the efficacy of ultrasonic energization in accelerating bone decalcification.
    • To evaluate the impact of ultrasonic agitation on hard and soft tissue morphology preservation.
    • To assess the compatibility of ultrasonic decalcification with various decalcifying agents and histochemical procedures.

    Main Methods:

    • Bone specimens (2-5 mm thickness) were subjected to decalcification using ultrasonic energization.

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  • Decalcification fluids included both acid and chelating agents.
  • Tissue morphology and the feasibility of histochemical staining were assessed post-decalcification.

    • Main Results:

    • Decalcification was completed in 5 hours or less with ultrasonic agitation.
    • Ultrasonic energization resulted in excellent preservation of both hard and soft tissue morphology.
    • Combined fixation-chelation protocols were compatible with ultrasonic decalcification, facilitating routine histochemical procedures.

    Conclusions:

    • Ultrasonic energization offers a rapid and effective method for bone decalcification.
    • The technique preserves tissue morphology, making it suitable for advanced histological and histochemical studies.
    • This method simplifies complex histochemical procedures previously requiring specialized handling.