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Experimental Proteus mirabilis burn surface infection

A T McManus, C G McLeod, A D Mason

Archives of Surgery (Chicago, Ill. : 1960)

|February 1, 1982

Summary

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This study shows that Proteus mirabilis causes lethal burn wound infections in rats. Bacterial motility is crucial for virulence, highlighting a new model for testing antimicrobial agents against Enterobacteriaceae wound infections.

Area of Science:

Microbiology
Pathogen Research
Infectious Diseases

Background:

Burn wound infections pose significant clinical challenges.
Enterobacteriaceae, including Proteus mirabilis, are common causes of hospital-acquired infections.
Understanding pathogen virulence factors is crucial for developing effective treatments.

Purpose of the Study:

To establish a rat model for experimental burn wound infection using a human isolate of Proteus mirabilis.
To investigate the role of bacterial motility as a virulence factor in this infection model.
To validate the model for evaluating antimicrobial agents against Enterobacteriaceae wound infections.

Main Methods:

Induction of scald injury in rats followed by surface inoculation with Proteus mirabilis.

Related Experiment Videos

Dose-response experiments to determine lethal inoculation doses.

Histopathological examination of infected tissues.

Investigation of bacterial motility using substrains with altered spreading capabilities.

Main Results:

A non-fatal scald injury significantly increased susceptibility to lethal Proteus mirabilis surface infection.
The lethal dose (50%) was less than 10^3 organisms/cm^2.
Infection progressed from surface colonization to tissue invasion, without specific perivascular/perineural lesions.
Bacteremia and high bacterial counts (>10^6 organisms/g) were observed in moribund animals.
Loss of bacterial motility (subsurface spreading) correlated with reduced virulence.

Conclusions:

Proteus mirabilis can act as an experimental pathogen causing lethal burn wound infections in rats.
Bacterial motility is a key virulence factor in this burn wound infection model.
This model is suitable for evaluating topical and parenteral antimicrobial agents for Enterobacteriaceae-related wound infections.