Three-dimensional subgross morphology of the human endometrium

A method is described for subgross, structural assessment of endometrial configuration. Human uteri from autopsy and surgical pathology were preserved in formalin and sectioned at 1-mm intervals. A procedure for staining, dehydration, and clearing was developed to achieve three-dimensional resolution of endometrial morphology. Processed specimens were sealed in translucent heavy plastic bags for the purpose of storage, easy manipulation under the dissecting microscope, and for photographic documentation. For simultaneous comparison with standard histological preparations, selected areas of sealed specimens were punched-out and paraffin sections made. This simple method can easily be applied to the future investigation and classification of focal premalignant, dysplastic, anaplastic, and atypical changes in the endometrium. The significance of this approach and its possible application to specimens obtained at dilatation and curettage is discussed.