Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural features and selective expression of three Ly-5+ cell-surface molecules

J S Tung, M P Scheid, M A Pierotti

    Immunogenetics
    |January 1, 1981
    PubMed
    Summary

    Researchers identified three cell-surface molecules associated with Ly-5 alloantisera. These molecules, differing between Ly-5.1 and Ly-5.2 cells, can be expressed independently by various hematopoietic cells.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The Lyb-2 phenotype of hemolytic PFC.

    Immunogenetics·2012
    Same author

    Biochemical demonstration- of anti-Qa activity in an H-2.28 public specificity antiserum.

    Immunogenetics·2011
    Same author

    Functional gamma-secretase inhibitors reduce beta-amyloid peptide levels in brain.

    Journal of neurochemistry·2001
    Same author

    Parent-progeny recognition as a function of MHC odortype identity.

    Proceedings of the National Academy of Sciences of the United States of America·2000
    Same author

    Effect of B2m gene disruption on MHC-determined odortypes.

    Immunogenetics·2000
    Same author

    Characterization of caspase processing and activation in HL-60 cell cytosol under cell-free conditions. Nucleotide requirement and inhibitor profile.

    The Journal of biological chemistry·1999

    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • The Ly-5 alloantigen system is crucial for distinguishing cell populations.
    • Conventional alloantisera identify Ly-5 associated molecules of 200K, 205K, and 220K sizes.

    Purpose of the Study:

    • To characterize the molecular forms precipitated by Ly-5 alloantisera and a specific monoclonal antibody.
    • To investigate the expression patterns of Ly-5 molecular forms in different cell types.

    Main Methods:

    • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze molecular sizes.
    • Cleveland mapping was employed to compare molecular structures.
    • Radiolabeling (125I) and precipitation assays were performed.

    Related Experiment Videos

    Main Results:

    • A rat monoclonal antibody (74/8') precipitated the same three molecular forms (200K, 205K, 220K) as conventional Ly-5 alloantisera.
    • Cleveland mapping revealed no differences within the three molecular forms but distinct differences between Ly-5.1 and Ly-5.2 associated molecules.
    • Cloned cell lines of Ly-5+ cells expressed these molecular forms independently, with most lines expressing only one form.

    Conclusions:

    • The Ly-5 alloantigen system involves at least three distinct molecular forms.
    • These forms exhibit allelic variation between Ly-5.1 and Ly-5.2.
    • Independent expression of these forms suggests complex regulatory mechanisms in hematopoietic cells.