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Insulin clearance by perfused rat lung

W A Stubbs, D M Kelly, I Morgan

Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme

|January 1, 1982

Summary

The rat lung clears insulin, with clearance rates varying based on the animal's metabolic state. Diabetic rat lungs showed significantly higher insulin clearance compared to fed or fasted states.

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Area of Science:

Physiology
Endocrinology
Pharmacology

Background:

Insulin is a crucial metabolic hormone regulating glucose homeostasis.
The lung's role in insulin metabolism is not fully understood.
Previous studies suggest potential extrahepatic insulin metabolism.

Purpose of the Study:

To investigate the capacity of the perfused rat lung to clear insulin.
To determine if insulin clearance is influenced by the animal's metabolic condition (fed, fasted, diabetic).

Main Methods:

Utilized a perfused in situ rat lung model.
Introduced porcine insulin at two concentrations (50 and 500 microunits/l) into the perfusion medium.
Measured insulin clearance rates (U/h) in lungs from fed, fasted, and diabetic rats.

Main Results:

Insulin clearance was observed in all tested conditions.
Diabetic rat lungs exhibited higher insulin clearance rates (15.7 U/h at 50 microunits/l) compared to fed (6.3 U/h) and fasted (6.1 U/h) rats.
Increased insulin concentrations led to higher clearance rates across all groups, with diabetic lungs showing the highest clearance (56.5 U/h at 500 microunits/l).

Conclusions:

The lung possesses a significant capacity for insulin clearance.
Insulin clearance by the lung is demonstrably dependent on the animal's metabolic state.
These findings suggest the lung plays a role in systemic insulin metabolism, particularly in diabetic conditions.

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