Complete amino acid sequence of the group-specific antigen gene-encoded phosphorylated proteins of mouse leukemia...

The complete amino acid sequence of the group-specific antigen gene-encoded RNA binding phosphoprotein p12 has been determined for both Rauscher and Moloney leukemia viruses. Large fragments generated by acid, and cyanogen bromide and hydroxylamine cleavage, and Staphylococcus aureus V8 protease digestion were subjected to automated sequencing. Both Rauscher and Moloney p12 are composed of 84 amino acids arranged in alternating variable and conserved regions. The homology between the conserved internal and COOH-terminal regions is greater than 95%, but the NH2-terminal and internal variable regions show 59 and 51% homology, respectively. The role of such regions in the type-specific biological activities of these molecules is discussed.