Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Codon selection in yeast

J L Bennetzen, B D Hall

    The Journal of Biological Chemistry
    |March 25, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Gene expression levels in yeast and E. coli are linked to codon bias. Highly expressed genes preferentially use specific codons that match major transfer RNA (tRNA) species, optimizing protein synthesis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Increase in CFC-11 emissions from eastern China based on atmospheric observations.

    Nature·2019
    Same author

    Recent trends in global emissions of hydrochlorofluorocarbons and hydrofluorocarbons: reflecting on the 2007 adjustments to the Montreal Protocol.

    The journal of physical chemistry. A·2014
    Same author

    DNA structures associated with autonomously replicating sequences from plants.

    Plant molecular biology·2013
    Same author

    Specific transcription and reinitiation of class III genes in wheat embryo nuclei and chromatin.

    Plant molecular biology·2013
    Same author

    Selection and characterization of cadmium-resistant suspension cultures of the wild tomato Lycopersicon peruvianum.

    Plant cell reports·2013
    Same author

    Genomic organization of the ribosomal DNA of sorghum and its close relatives.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
    Same journal

    Wanted and unwanted modifications of mRNA, and their effect on gene expression and signaling.

    The Journal of biological chemistry·2026
    Same journal

    TGF-β2 drives lipid droplet accumulation in chondrocytes through the TβRI/p-Smad3/Fabp5 axis.

    The Journal of biological chemistry·2026
    Same journal

    Macrophage-specific targeting of histone demethylases with small-molecule inhibitors suppresses inflammatory response in vivo.

    The Journal of biological chemistry·2026
    Same journal

    Substrate and target selectivity of 4'-fluoroadenosine against viral and host polymerases.

    The Journal of biological chemistry·2026
    Same journal

    Correction: Characterization of Mast2 kinase defines structural features, regulation, and substrates.

    The Journal of biological chemistry·2026
    Same journal

    Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

    The Journal of biological chemistry·2026
    See all related articles

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biochemistry

    Background:

    • Codon bias, the non-uniform usage of synonymous codons, is observed in many organisms.
    • This bias is thought to be influenced by factors such as tRNA availability and translational efficiency.
    • Understanding codon usage patterns is crucial for comprehending gene expression regulation.

    Purpose of the Study:

    • To investigate the extreme codon bias in Saccharomyces cerevisiae genes, specifically alcohol dehydrogenase isozyme I (ADH-I) and glyceraldehyde-3-phosphate dehydrogenase.
    • To determine the relationship between codon bias and gene expression levels in yeast and Escherichia coli.
    • To compare codon preferences between yeast and E. coli and identify potential incompatibilities.

    Main Methods:

    • DNA sequencing to analyze amino acid residues and identify preferred codons.

    Related Experiment Videos

  • Analysis of codon-anticodon homology with major isoacceptor tRNA species.
  • Correlation analysis between codon bias and mRNA levels in the cytoplasm for various genes.

    • Main Results:

    • Yeast genes ADH-I and glyceraldehyde-3-phosphate dehydrogenase exhibit extreme codon bias, using only 25 out of 61 possible codons.
    • Preferred codons are homologous to major yeast isoacceptor tRNA anticodons, avoiding GC base pairs and extreme base compositions.
    • The degree of codon bias in yeast genes correlates directly with their mRNA levels, with highly expressed genes showing greater bias. A similar phenomenon is observed in E. coli.

    Conclusions:

    • Codon bias in highly expressed genes is a conserved mechanism across yeast and E. coli, optimizing translational efficiency.
    • The selection of preferred codons is driven by complementarity to major tRNA species, ensuring efficient protein synthesis.
    • Differences in preferred codons exist between yeast and E. coli, indicating species-specific adaptations in translational machinery.