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Related Experiment Videos

Defective cellular immune response in vitro in common variable immunodeficiency

S Cunningham-Rundles, C Cunningham-Rundles, F P Siegal

    Journal of Clinical Immunology
    |January 1, 1981
    PubMed
    Summary

    Patients with common variable hypogammaglobulinemia exhibit impaired lymphocyte proliferation to mitogens and microbial antigens. This consistent deficiency suggests intrinsic B-cell functional defects impacting immune responses.

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    Burden of copy number variation in common variable immunodeficiency.

    Clinical and experimental immunology·2013

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Common variable hypogammaglobulinemia (CVID) is a primary immunodeficiency characterized by low immunoglobulin levels.
    • Understanding lymphocyte function in CVID is crucial for diagnosing and managing the condition.

    Purpose of the Study:

    • To investigate the in vitro proliferative responses of lymphocytes from CVID patients to various mitogens and microbial antigens.
    • To assess the capacity of CVID patient lymphocytes to generate specific secondary immune responses.

    Main Methods:

    • Mononuclear cells from 39 CVID patients and controls were analyzed for proliferative response to phytohemagglutinin, concanavalin A, pokeweed mitogen, zinc, Candida albicans, Escherichia coli, and Staphylococcus aureus.
    • Repeated testing was performed to assess consistency of responses.

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    Main Results:

    • CVID patient lymphocytes showed significantly lower proliferative responses to phytohemagglutinin, concanavalin A, and pokeweed mitogen compared to controls (P < 0.01).
    • Responses to microbial antigens (Candida albicans, Escherichia coli, Staphylococcus aureus), which require intact B-cell function, were also significantly depressed (P < 0.01).
    • Low responses did not correlate with T-lymphocyte numbers, and responses to zinc were normal in a few patients tested.

    Conclusions:

    • Patients with CVID demonstrate a consistent pattern of intrinsic lymphocyte functional deficiency.
    • The impaired response to microbial antigens suggests defects in B-cell function contributing to the immunodeficiency in CVID.